“雞巨噬細胞替代激活相關化學因子1試劑盒”供應商的:
研發,批量生產,具有省時,質優價廉
采用純進口試劑原料和進口工藝流程批量生產
快速
質量穩定
出廠經全套質控程序(標準曲線線性質控、37度穩定性試驗、批次間、批次內重復性質等)
現貨快速,到貨及時,采用信譽好的快遞,從出庫到客戶地址,客服及時跟蹤
售后及時,齊全,技術指導.
“雞巨噬細胞替代激活相關化學因子1試劑盒”性能:
1. 準確性:標準品線性回歸與預期濃度相關系數R 值,大于等于0.9900。
2. 靈敏度:zui低檢測濃度小于1.0 pmol/L。
3. 特異性:不與其它可溶性結構類似物交叉反應。
4. 重復性:板內、板間變異系數均小于15%。
5. 貯藏:2-8℃,避光防潮保存。
6. 有效期:6 個月
Important notes
1. The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.
2. washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilute . Washing does not affect the result.
3. add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 min, if the number of sample is much , recommend to use Volley .
4. if the testing material content is excessively higher (The sample OD is bigger than the first standard well ),please dilute Sample (n-fold), Please diluente and multiplied by the dilution factor.(×n×5).
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5. Closure plate membrane only limits the disposable use, to avoid cross-contamination.
6. The substrate evade the light preservation.
7. Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.
8. All samples, washing buffer and each kind of reject should according to infective material process.
9. Do not mix reagents with those from other lots.
Storage and validity
1.Storage: 2-8℃.
2.validity: six months.
要步驟為以下幾點:
1、盡量用八道或者十二道移液器加樣。對于相同的試劑,經如二抗,酶,顯色液,終止液等。
2、自己的樣品,因為每一孔的樣品不一樣,用排槍加有些麻煩,如果一次的樣品比較少,而自己加樣又比較快,可以一個孔一個孔的加,但如果想一次把96或者48T的做完,可以先擺好一些PCR管或者準備一個96孔細胞培養板。將自己的樣品先加到PCR管或者培養板中(此板間距與酶板板相同)。記得加一定的余量,如一次加樣是50ul,可以先加70ul。加完后再用排槍加樣,這樣可以縮短加樣時間,減小每個孔間的時間誤差。
3、如果有多余的孔,標準品孔可以做復孔,在自己的樣品前加一次標準品,加完樣品后再加一次標準品。如果孔不夠,那么可以將標準品放在樣品中間加。
公司ELISA試劑盒款到當天發貨,代測,一周內出結果。
上海勁馬生物科技有限公司所銷售的“雞巨噬細胞替代激活相關化學因子1試劑盒”,涉及的品牌R&D、R&B等美國原裝進口不同價格檔次的ELISA試劑盒。
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